Emission. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Includes: SpectraMax 340PC384. 4. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 5. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. \r plates can be used in the SPECTRAmax PLUS 384. ATI/240, 250. ELISA plates were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). SpectraMAX 340. , Sunnyvale, Calif. One plate carrier adapter is provided with the instrument. 0 2. Immunofluorescence and Aggregation Analysis. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. 0. Anti-proteolytic activity. 5. Molecular Devices Spectramax 340PC instructions. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. P. All data were normalized to experimental day 0. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). Wells were washed three times. mums. (1993). 5 mL of 1 M acetate buffer (pH 4. 316 grating motor driver faulty. TheSpectraMax®Plus384addstheabilitytoreadcuvettes. Cell proliferation was measured using the MTT method. 303 unable to cal dark current. The ODs of wells loaded with DMEM plus 10% CS were subtracted as background. using a Molecular Dynamics Model SpectraMAX 340 PC microplate reader. The values were corrected for nonspecific background by subtracting the OD 650. Hydrolysis of compounds 9-15 was monitoredAbsorption was measured at 405 nm against 490 nm using a microplate reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). more on but is otherwise untested and sold AS-IS. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). (Shiga, Japan) and kept for 30 min in the dark. Page: 1/6. Details. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Briefly, cells were seeded into a. The area under the curve (AUC) for the comparison of the growth rates was determined using. Table 1. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. Each experiment was performed in duplicate and repeated at. Quick View. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature. Bertin Precellys Evolution Touch Homogenizer. With SOFTmax PRO, the contents of the wells in a microplate can be mixed SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. One plate carrier adapter is provided with the instrument. edu Contact Phone: 216-368-8962. In vivo anti-malarial assay in mice. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). Manager: Yinghua Chen, PhD Contact Email: yinghua. Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. Near bottom. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. This is equivalent to having 511 built-in filters,Colour change was measured in a SpectraMAX ® 340 spectrophotometer. TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Donor: 620 nm. The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. The concentration of TNFα in the experimental samples was calculated from a mouse TNFα standard curve of 15–2000 pg/ml. 305 reference level saturation. SpectraMAX 340. D. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. IMAP ® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. 340/70 nm. Details. 2 software was used to measure and calculate of the bioactivity activity studies. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The VersaMax and SpectraMax 190 read 96-well microplates. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. density was measured at 570 nm (SpectraMax 340, Molecular De-vices, Sunnyvale, CA). Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. MMP‑1 inhibition assay. Labsystems Luminoskan RT Microplate Reader. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. Manager: Yinghua Chen, PhD Contact Email: yinghua. 30. PerkinElmer. 1 0 100 80 60 40 20 0 4. Analytical Data Regression and Acceptance Limits The conversion of the optical density units for the test samples, VSs, and QCs to concentrations was performed using the Watson LIMS software (version. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The amount of liberated pNA product during incubations was determined using a standard curve obtained at 410 nm for various concentrations of a pNA stock solution in the SpectraMax 340 microplate reader. Values were determined according to a standard curve (log/log curve-fit). When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). Molecular Devices Spectramax 340 Plate Reader Repair. 0. 30. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. Glutathione reductase (GR) activity was measured, according to Smith et al. Gerritsma,B. Features showcased by this model include. Each SpectraMax® 340 PC 384 Microplate Reader has an. 024). Statistical analysis. Driessen*,MaaikeA. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Service parts for these models are not being produced and are no longer in stock. The slope of the linear portion of the kinetic curve generated from each well was used to. Operators can conveniently select up to 6 wavelengths at a time for absorbance detection in the visible wavelength range 340 nm - 850 nm. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models article for additional information. FACS analysis. Male 8 pin mini Din to Female 9D. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . Triton X-100 (see Note 2). automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. The protein concentration was determined spectrophotometrically at 280 nm in a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis spectrophotometer. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. Intra- and inter-assay coefficients of variation were lower than 8. Cell culture 3T3 fibroblasts and RAW 264. Statistical analysis The significant differences between the different strains in the adherence assays were analyzed using the Student’s t -test. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). Recalcification (clotting) time was determined by monitoring absorbance at 650 nm at 10-sec intervals in a SpectraMax 340 microtiter plate reader, with onset time (the time to a linear increase in the OD, which reflects the maximal rate of formation of insoluble fibrin) set at an OD of 0. Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. The SPECTRAmax 340PC can mea- TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes acrossawiderangeofconcentrations. , Sunnyvale, CA). The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. On-site Repair Service available nationwide for Molecular Devices Spectramax 340 Plate Reader's. Money Saved: $340. The. Other technologies such as imaging. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). Microplate Readers. Western blot. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial growth, & protein quantitation. SpectraMax 340 (Obsolete), SpectraMax 340PC 384 (Obsolete), Gemini EM reader, SpectraMax 250 (Obsolete) What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full. The absorbances were transformed to velocity of substrate consumption (nmol/min). , Sunnyvale, CA). The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Fully tested with Warranty. Briefly, cells were seeded into a. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. SoftMax Pro. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. well, and strip-well microplates. Also for: Spectramax 190, Spectramax 340pc384, Versamax. About The SpectraMax GEMINI EM is controlled by an external computer running SoftMax ® Pro software which provides integrated instrument control, data display, and statistical data analysis. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. 003 OD Stray light ≤ 0. ). Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. TDP-43 stress granular cells were treated with 70% ice-cold. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Microplate Readers. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. The extreme flexibility and high sensitivity of the SpectraMax GEMINI EM make it appropriate for applications within the fields of bio-A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). For 40 years, we have partnered with scientists to help them achieve landmark discoveries. control. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. The plate was covered and incubated for one hour at room temperature. SpectraMax Gemini EM & FLEXstation (bottom read) 1. 4. . control. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). edu Contact Phone: 216-368-8962. ). SpectraMax QuickDrop Micro-Volume Spectrophotometer. #N207) LAL Reagent Water (Lonza cat. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. The chamber contains. This was followed by an additional incubation at 37 °C for 30–60 min. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. ir Neuroprotection of a sesamin derivative, 1, 2-bis [(3-methoxy- phenyl) methyl] ethane-1, 2-dicaroxylic acid (MMEDA) againstEach SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. Following HRP-conjugated secondary antibodies, goat anti-rat IgG (Kirkegaard & Perry. Bioz Stars score: 86/100, based on 1 PubMed citations. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Molecular Devices SpectraMax Gemini XS Microplate Reader. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. 2 were considered negative for IgM antibody. Molecular Devices’ PathCheck® sensor technology. 0% and ± 0. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Quick View. About GMI provides new and GMI Certified Pre-Owned scientific instrumentation and service to research universities, environmental labs, forensic investigators, biotechnology and pharmaceutical companies, hospitals, clinics and analytical laboratories, in the United. Wavelength Selection. 2. Samples were considered “positive” if the absorbance value was greater than two times the average absorbance value of the negative control wells . plates can be used in the SPECTRAmax PLUS 384. albicans pre. They are for reference only. The control panel consists of a 2-x-20-character LCD and eleven pressure-sensitive membrane keys that can be used to control some functions of the instrument. Each experiment was performed in duplicate and repeated at. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Filterless readers provide consistent results with steady temperature regulation. Subsequently, the mixture was heated at 100 °C for 10. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). Labsystems Luminoskan RT Microplate Reader. This light offers spectrum variability, wireless control, and photosensors to automatically balance output with natural light. 1 All Necessary Cables and Cords Manuals General Specifications Dimensions (in. The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. 04. 313 reference gain check fail. edu Contact Phone: 216-368-8962. These models can no longer be repaired. These lab workhorses are used in drug discovery, research, bioassay validation, and. 4. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating. 1 mL of culture media with 10% FBS. 00 USD. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). Fully tested with WarrantyThe SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). A 0. , Oxon, UK). As a common indicator of equine T cell activation, cytokine induction. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). StakMax Microplate Handling Stacker. 303 unable to cal dark current. 7. Optical density was read at 405 nm after 30 min using a Molecular Devices SpectraMax 340 spectrophotometer. Wavelength(s) 340 to 850 nm; Add to Compare List. 0% and ± 0. The metals were preincubated with A for 5 min when added before heme. The absorbance was measured at 550 nm with a Spectramax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. 304 signal level saturation. Laser Scanning Confocal Microscopy The fluorescent signals were obtained using a Zeiss LSM 510 laser scanning confocal microscope. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. 315 can't find zero order. No power cord or accessories included. Not. Data are expressed as percent of maximum binding for each protein. Acceptor filter: 665 nm. Number of flashes. The screening was initiated by adding 10 μL p-nitrophenyl phosphate (pNPP), and the catalysis of pNPP (1 mM) was continuously monitored on SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at 405 nm for 20 min at 37 °C. Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). Thin layer chromatography (TLC) was. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. SpectraMax® Microplate Readers and SoftMax® Pro Software. Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). The plates were incubated for 4 additional hours at 37°C. 5. MOLECULAR DEVICE M5. 81. The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs. chen@case. 0, 20 mmol L –1cal-cium chloride). Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). application or budget. , Fremont, CA, USA). The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. Studies of the regulation of AoSMC migration by Angpt2 were preformed using a wound healing assay. Stat Fax 3200 Microplate Reader. SpectraMax Multi-Mode Microplate Readers. The mixture was centrifuged for 10 min at 10,000 rpm, and BSA concentration in the supernatant was determined using bicinchoninic acid (BCA) assay. SpectraMax Multi-Mode Microplate Readers. AoSMCs were seeded into 6-well culture plates. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. 5 mL of 1 M acetate buffer (pH 4. The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. Call 1-800-745-2710. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. Introduction. The extinction coefficient at 280 nm used for determining the concentration of Rsp3690 was calculated to be 28,795 M –1 cm –1. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). #ABS PLUS) Methods. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 2. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. Microplate Readers. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. Microscopy. Detection limits. 340 nm. 6 (h) x 22. Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). Clotting times (mean ± SD) for rSimukunin and rSV. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. 6. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. All ELISA plates were analyzed using a SpectraMax 340 PC microplate reader (Molecular Devices). using SpectraMax i3 (Molecular devices, San Jose, USA). g. Manager: Yinghua Chen, PhD Contact Email: yinghua. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. Details. measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Or scan up to 384 wells at any time to determine the best assay conditions. MSD read buffer and TMB one component HRP substrate are from MSD and SurModics (Eden Prairile, MN), respectively. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. Product Keyword. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. A 0. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. Immunofluorescence and Aggregation Analysis. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. The ABS reader ranges from. Dissolve 13. 1. using SpectraMax i3 (Molecular devices, San Jose, USA). 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). All assays were done in triplicate with readings at 60-s intervals using a SpectraMax 340 multiwell plate reader (Molecular Devices, San Jose, CA, United States). 5% phosphoric acid) in microplates at room temperature for 10 min. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 2 Chemical Reagents 1. Percent Transmission Absorbance Competitor 0. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. 5. One plate carrier adapter is provided with the instrument. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. ac. 5. Awareness Technology Inc. 2. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Emission. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. The growth. Customer Transformation Hub. Please see photos. 6. Molecular Devices SpectraMax M5e Microplate Reader. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology.